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HOME Lab and Medical Equipment Categories Chromatographs, HPLC Fluorescence Detectors Agilent HP 1200 / G1321A

Agilent HP 1200 / G1321A

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Description of Agilent HP 1200 / G1321A

Categories: Chromatographs, HPLC, Fluorescence Detectors

Manufacturer: Agilent

Condition: Used

Seller: Conquer Scientific, LLC

Agilent 1200 G1321A FLD

Condition: Used

AGILENT 1200 G1321A FLD FLUORESCENCE DETECTOR

This user-friendly detector brings high sensitivity fluorescence detection for you needs whether used alone or in an automated Agilent 1200 Series system. One run will provide quantitative data and fluorescence spectra all the while improving sensitivity and selectivity with the simultaneous multi-wavelength detection capabilities.

The detector is designed for highest optical performance, GLP compliance and easy maintenance. It includes the following features:
  • Superior sensitivity for lower detection limits at any combination of excitation and emission wavelengths.
  • Improved selectivity and sensitivity by simultaneous multiple wavelength detection.
    • Monochromators work in signal or spectral mode and are synchronized with the flash light to allow for detection at up to 4 excitation or emission wavelengths.
  • Dual wavelength operation now available.Method optimization or peak confirmation by exploring the spectral landscape.
  • Multitask viewing online spectra without compromising your chromatography.
  • Fast offline characterization of new compounds -
    • Fill the FLD cuvette with your pure compound and take a fluorescence scan to get a complete visualization of all excitation and emission spectra with a single task.
  • Verify wavelength accuracy for GLP compliance - Automatically check the built-in wavelength calibration (Raman band H20) with external standards.
  • Easy front access to flow cell for fast inspection or exchange.
Specifications:
Specifications Agilent 1200 Series Fluorescence Detector
Performance Single wavelength operation: 10 fg anthracene LOD, Ex 250 nm, Em 400 nm H20 >500 Raman, Ex 350 nm, Em 397 nm, dark value 450 nm, standard flow cell Dual wavelenght operation: H20 > 300 Raman, Ex 350 nm, Em 397 nm and Ex 350, em 450 nm, standard flow cell
Light source 20 W Xenon flash lamp, normal mode: 20 W economy mode: 5 W
Pulse frequency 365 Hz for signal mode, 73 Hz for economy mode
Excitation monochromator Concave holographic grating, F/1.6, blaze 300 nm, range 200-700 nm and zero-order, bandwidth 20 nm
Emission monochromator Concave holographic grating, F/1.6, blaze 400 nm, range 280-900 nm and zero-order, bandwidth 20 nm
Photomultiplier 200-600 nm, exchangeable
Reference system Inline excitation measurement
Time programing Up to four signals, response time, PMT gain, baseline behavior (append, free, zero), spectral parameters
Spectral acquisition Excitation or emission spectra, scan speed 28 ms per datapoint, e.g. 0.6 s per spectrum 200-400 nm, 10 nm step
Step size 1-20 nm
Spectra storage All
Wavelength repeatability ±0.2 nm
Wavelength accuracy ±3 nm
Flow cells Standard: 8 µL volume, 20 bar (2 MPa) pressure maximum, quartz. Optional: fluorescence cuvette for offline spectroscopic measurements with 1 mL syringe, 8 µL volume, quartz
Control and data evaluation Agilent ChemStation for HPLC or Agilent 1100 Series control module without spectral data analysis
Environment 0 - 40 °C constant temperature at <95% humidity (non-condensing)
GLP Early maintenance feedback (EMF) for continuous tracking of instrument usage, display of feedback messages in terms of lamp burn time with user-settable limits and feedback messages. Electronic records of maintenance and errors. Verification of wavelength accuracy using Raman band (H20) lines.

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