Experience rapid cell counting with the Countess 3 Automated Cell Counter. Equipped with advanced optics, automated focus and lighting, and AI-based image analysis software, the Countess 3 brightfield cell counter enables automated cell counting and measurement of cell viability.
The Countess 3 Automated Cell Counter is a brightfield cell counter equipped with an advanced machine-learning algorithm, ruggedized optics, fully-automated focus and lighting, and image analysis software. This benchtop cell counting system is designed for rapid automated cell counting and viability measurement of primary and immortalized cell samples to prepare cells for downstream analysis and for routine cell culture passaging.
Features of the Countess 3 Automated Cell Counter include:
- Accuracy—highly accurate machine-learning algorithm applied to automated cell counting, eliminates the subjectivity of manual cell counting and user-to-user variability
- Time savings—counts live and dead cells, measures viability, calculates percent cell aggregates, and reports average cell size with a single touch in about 20 seconds
- Convenience—requires no cleaning, routine maintenance, or calibration; flush-mounted high-resolution capacitive touchscreen and simple user interface provide quick startup, require minimal training, and offer automatic saving of data to USB or cloud
- Flexibility—compatible with both disposable and reusable cell counting chamber slides
The Countess 3 Automated Cell Counter works in two simple steps:
- Mix 10 μL of sample with 10 μL of trypan blue and pipet into a disposable or reusable Countess chamber slide.
- Insert the slide into the instrument. Rapid Capture and Auto-Save modes allow automated cell counting with a single touch. Focus, lighting, and file saving are executed automatically.
Obtain accurate cell counts
The Countess 3 Automated Cell Counter uses trypan blue staining combined with auto-focus and lighting driven by a machine-learning image analysis algorithm to obtain accurate cell and viability counts. The measurement range extends from 1 x 104 to 1 x 107 cells/mL (the optimal range is 1 x 105 to 4 x 106 cells/mL), which is broader than that of a hemocytometer.
Transfer data and images to your PC or cloud
Using the USB port on the Countess 3 Automated Cell Counter and any USB drive, you can save your cell count results and images and transfer them to your PC. For your convenience, a USB drive is supplied with the Countess 3 instrument, and a WiFi dongle is available.
Improve downstream single-cell analysis
Minimizing cell aggregates is crucial for RNA sequencing applications to ensure that individual cells are partitioned and barcoded during the single-cell library preparation process. For optimal single-cell data output, the percent cell aggregates on the Countess 3 Automated Cell Counter can be used to confirm that the starting sample has <5% aggregates before performing downstream single-cell analysis