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Solvent Management |
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Solvent Conditioning |
Vacuum degas, three solvents plus purge solvent |
Typical Operating Flow Rate Range |
0.050 to 5.000 mL/min in 0.001-mL/min increments |
Programmable Flow Rate Range |
.000 and 0.010 to 10.000 mL/min in 0.001-mL/min increments |
Compressibility Compensation |
Automatic and continuous |
System Delay Volume |
<400 µL, independent of backpressure, 1 mL/min |
Plunger Seal Wash |
Integral, active, programmable
Gradient Profiles: Eleven (11) gradient curves [including linear, step (2 curves), concave (4 curves), and convex (4 curves)] |
Gradient Profiles |
Eleven (11) gradient curves [including linear, step (2 curves), concave (4 curves), and convex (4 curves)] |
Dry Prime/Wet Prime |
Automatic, front panel control |
Flow Ramping |
Time (0.01 to 30.00 min in 0.01-min increments) to reach maximum flow rate |
Maximum Operating Pressure |
5000 psi (345 bar) at 0.010 to 3.000 mL/min; Programmable upper and lower limits |
Composition Range |
0.0 to 100.0% in 0.1% increments
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Composition Accuracy |
±0.5% absolute, independent of backpressure (Proportioning Valve Pair Test [degassed methanol: methanol/propylparaben, 2 mL/min, 257 nm]) |
A Composition Precision
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<=0.15% RSD or<=0.02 min SD, whichever is greater, based on retention time. (Degassed 60% methanol:40% water Dial-a-Mix, 1 mL/min, six replicates, phenone mix, 257 nm) |
Flow Precision |
<=0.075% RSD or <=0.02 min SD, whichever is greater, based on retention time (N = 6) or volumetric measures (0.200 to 5.000 mL/min), isocratic premix |
Flow Accuracy |
<=1% or 10 µL/min, whichever is greater, (0.200 to 5.000 mL/ min), degassed methanol, at 600 psi backpressure |
Sample Management |
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umber of Sample Plates: |
Total of four plates:
- Microtiter plate – 96- or 384-well
- Vial plate – 2.0-mL vial (48)
- Tube plate – 0.5-mL microcentrifuge tube (48) or 1.5-mL microcentrifuge tube (24)
- Open access plate – 2.0-mL vial (24 or 48) |
Sample Temperature Control: |
4 to 40 °C, programmable in 1 °C increments |
Maximum Sample Capacity |
1536 in four 384-well plates |
Number of Sample Injections: |
1 to 99 injections per sample |
Sample Delivery Precision: |
<0.3% RSD, full loop 50 µL. (Default wash/purge conditions, degassed 60% methanol: 40% water Dial-a-Mix, 1 mL/min, six replicates, paraben mix, 257 nm) |
Sample Volume Linearity |
>0.999% correlation, 5 to 20 µL, partial fill in 50-µL loop, 3-µL pre- and post-sample air gaps (Default wash/purge conditions, degassed 60% methanol:40% water Dial-a-Mix, 1 mL/min, six replicates, paraben mix, 257 nm) |
Needle Wash Solvents |
Two: wash (strong solvent) and purge (sample- compatible) solvent A |
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